特(te)色:
可用于(yu)檢測 R 環
對 DNA-RNA 雜交體(ti)的高特異性和親(qin)和力
不與單鏈(lian) DNA 或雙鏈(lian) DNA 發(fa)生交叉反(fan)應
對于(yu)富含 AU 的雙鏈 RNA,觀察到(dao)了輕微的交叉(cha)反應(約 5 倍以下)。
長(chang)度為 8、10、15 和(he) 23 個堿基對的雜交(jiao)體顯示出高親(qin)和(he)力結(jie)合
DNA-RNA 雜合體(ti)是(shi)真核細(xi)胞中的(de)一種自然現(xian)象,這些(xie)雜合體(ti)的(de)水平在具有(you)高(gao)轉錄活性(xing)的(de)位點增加,例如在轉錄起始、抑制(zhi)和延伸(shen)期間(jian)。由于(yu) RNA-DNA 雜合體(ti)會影(ying)響基(ji)因(yin)組的(de)不穩定性(xing),因(yin)此 S9.6 抗(kang)體(ti)是(shi)一種有(you)用的(de)試劑,可幫助研究(jiu)在 DNA 復制(zhi)或其他細(xi)胞過程中由這些(xie)雜合體(ti)形成的(de) R 環和損傷的(de)后果。此外(wai),S9.6 抗(kang)體(ti)可有(you)效識別(bie)用于(yu)微陣列研究(jiu)的(de) RNA-DNA 雜交。
This mouse monoclonal antibody was generated against a ΦX174 bacteriophage-derived synthetic DNA–RNA antigen and recognizes RNA-DNA hybrids of various lengths.
Highlights:
* Useful in the detection of R-loops
* High specificity and affinity for DNA-RNA hybrids
* Does NOT cross-react with single-stranded DNA or double-stranded DNA
* Minor cross-reaction (~5-fold less) has been observed for AU-rich double-stranded RNA.
* High affinity binding shown for hybrids of 8, 10, 15, and 23 base pairs in length
產品詳情:
Product Type: | Antibody |
Name: | Anti-DNA-RNA Hybrid [S9.6] |
Antigen: | S9.6 ΦX174 bacteriophage-derived synthetic DNA–RNA antigen |
Isotype: | Rabbit IgG |
Fusion Tag(s): | Mouse Fab version contains His-tag |
Clone Name: | S9.6 |
Reactivity: | High specificity and affinity for DNA/RNA hybrids and other A-form nucleic acid hybrids |
Immunogen: | ΦX174 bacteriophage-derived synthetic DNA/RNA |
Purification Method: | Protein A/G |
Buffer: | ENHOO1: PBS, 0.05% (w/v) Sodium Azide Ab01137- : PBS with 0.02% Proclin 300 |
Tested Applications: | Dot Blot Analysis: 0.2 µg/mL. |
參(can)考文(wen)獻(xian):
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