您是(shi)否希望懸浮細(xi)(xi)胞(bao)(bao)(bao)的(de)(de)免(mian)疫(yi)熒光像貼壁細(xi)(xi)胞(bao)(bao)(bao)一樣(yang)容(rong)易?現在(zai)是(shi)這樣(yang)!Shi-fix™(由Shikhar生物技術公司制造(zao))蓋滑(hua)允許(xu)懸浮細(xi)(xi)胞(bao)(bao)(bao)分層或(huo)直接作為(wei)單層生長。簡單,無憂的(de)(de)實驗方(fang)案,無需離心柱即可(ke)將懸浮細(xi)(xi)胞(bao)(bao)(bao)固定到載玻(bo)片上。只需將細(xi)(xi)胞(bao)(bao)(bao)添(tian)加(jia)到蓋玻(bo)片中,等(deng)待30分鐘,用PBS洗滌未結合(he)的(de)(de)細(xi)(xi)胞(bao)(bao)(bao)并繼續免(mian)疫(yi)染(ran)色(或(huo)繼續培養以獲得所需的(de)(de)細(xi)(xi)胞(bao)(bao)(bao)密(mi)度)。
這些蓋玻(bo)片也(ye)可用(yong)于染色懸(xuan)浮細(xi)胞(bao)(bao)以(yi)進行(xing)顯(xian)(xian)微鏡檢查,或用(yong)于固定懸(xuan)浮細(xi)胞(bao)(bao)以(yi)進行(xing)共(gong)聚焦顯(xian)(xian)微鏡檢查。更(geng)重要(yao)的是,如果需要(yao),您還可以(yi)在細(xi)胞(bao)(bao)附(fu)著在蓋玻(bo)片上(shang)時(shi)刺激它(ta)們。您可以(yi)像(xiang)貼(tie)壁(bi)(bi)細(xi)胞(bao)(bao)一樣處理非貼(tie)壁(bi)(bi)細(xi)胞(bao)(bao)!
Do you wish immunofluorescence of suspension cells was as easy as with adherent cells? Now it is! Shi-fix™ (manufactured by Shikhar Biotech) cover-slips allow suspension cells to be layered on them or directly grown as a monolayer. Easy, hassle-free protocol and no spin columns needed for fixing suspension cells to slides. Just add your cells to the coverslips, wait for 30 mins, wash unbound cells with PBS and proceed to immunostaining (or continue the culture to obtain desired cell densities).
These cover-slips can also be used for staining suspension cells for microscopy, or for fixing suspension cells for confocal microscopy. What’s more, you can also stimulate the cells while they are attached on the cover-slip, should you need to do so. You can work on non-adherent cells as if they were adherent!
SHI-FIX cover-slips玻片做的懸浮細胞免疫熒光實驗圖:
SHI-FIX cover-slips玻片的操作步驟:簡單!方便!!
SHI-FIX cover-slips玻片的產品訂購信息: