Emfret公司血小板消除抗體R300說明書
Antibodies for Platelet Depletion in Mice
# R300 |
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產品資料 |
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貨(huo)號 | R300 |
同種型(xing)(isotype) | 大鼠(shu)IgG (Wistar) |
含量 | 0.5 mg purified immunoglobulins in PBS, sterile filtered |
包裝(zhuang) | 1ML |
濃度(du) | 0.5 mg/ml |
本產品只用于科研,不用于診斷,也不屬于醫療用品
特異性: This antibody preparation contains a mixture of purified rat monoclonal antibodies directed
against mouse GPIba (CD42b). Targeting of this receptor with divalent IgGs results in profound and
irreversible Fc-independent plaet depletion in mice1,2. Plaet depletion is frequently used to study
the general role of plaets in (patho-) physiology3.
制備 : 抗體是雜交瘤細胞上清通過G-Sepharose純(chun)化所得,內(nei)毒素含量小于 0.0025 EU/µg
保存和運輸:4度保(bao)(bao)(bao)存(cun)(cun)和運(yun)輸,保(bao)(bao)(bao)質期為(wei)6個(ge)月 ,無菌保(bao)(bao)(bao)存(cun)(cun)不(bu)含防腐劑,分裝保(bao)(bao)(bao)存(cun)(cun)-20度至少可以保(bao)(bao)(bao)存(cun)(cun)一年以上,但請避(bi)免(mian)反復凍融(rong)
使用(yong)方法: 按動物體重注射抗體,用 量為2 µg抗體/g動物體重,可以參見上圖。如果4 µg抗體/g動(dong)物體重,可(ke)以持續更長時間(jian)。注射后(hou)60分鐘內可以去除血小板95%以上,建議每次注射100-200µL抗體(用無菌PBS稀釋)。詳情聯系靶點科技技術支持。可以聯合清除巨噬細胞(LIPOSOMA,CP-005-005)和中性粒細胞,咨詢綜合解決方案。
引用文獻:
1. Bergmeier W, Rackebrandt K, Schroder W, Zirngibl H, Nieswandt B. (2000) Structural and functional characterization of the mouse von Willebrand
factor receptor GPIb-IX with novel monoclonal antibodies. Blood. 95:886-93.
2. Nieswandt B, Bergmeier W, Rackebrandt K, Gessner JE, and Zirngibl H. (2000) Identification of critical antigen-specific mechanisms in the
development of immune thrombocytopenic purpura in mice. Blood. 96:2520-27.
3. Elzey BD, Tian J, Jensen RJ et al. (2003) Plaet-mediated modulation of adaptive immunity. A communication link between innate and adaptive
immune compartments. Immunity.19:9-19.